So just to confirm.....you are trying to transfer a single file that is 400GB in size?
What are the exact make & models of these two USB drives? Provide a link to their product page if you can.
What file systems are each of these two drives?
Are these drives connected to the USB-A or USB-C ports? Since you mentioned USB3 ports.....I assume you mean the traditional USB-A ports?
Have you tried booting into Safe Mode in order to attempt to transfer the file?
You should also try running Disk Utility First Aid on both external drives. Even if the First Aid summary says everything is "Ok", click "Show Details" and scroll through the report to see if there are any unfixed errors or warnings. If so, then run First Aid again until they are gone, but after several scans the errors remain, then they cannot be repaired by First Aid. If either one is using the APFS file system, then run First Aid on the hidden Container which can be revealed by clicking "View" within Disk Utility & selecting "Show All Devices".